Composite

Part:BBa_K823040

Designed by: Julia Bartels   Group: iGEM12_LMU-Munich   (2012-09-10)

Inverter: pBad-RyhB-pLac(R0011)-uof-lacZalpha

Inverter pellets.jpg

This part reverses the positive Input of the PBAD Promoter (only short form without upstream Repressor binding site) induced by Arabinose to a negative output of the lacZα Reporter, by the translational inhibitory action of the small RNA RyhB to uofCGU translationally fused to lacZα.

Inverter graph.pngInverter Construct.png

This Image depicts the β-Galactosidase assay done with this part. It converts the positive Input of the PBAD promoter induced by Arabinose into an negative output, therefore declining Miller Units with rising Arabinose concentration (as Repressor binding sites are missing, the output without Arabinose is not so high, as Promoter is leaky).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 495
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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